MAP1ALC3,cleaved (LC3B,MAP1LC3B,Microtubule-associated proteins 1A/1B light chain 3B,Autophagy-related protein LC3 B,Autophagy-related ubiquitin-like modifier LC3 B,MAP1 light chain 3-like protein 2,MAP1A/MAP1B light cha

Antibodies / Antibodies-Microtubule-associated Proteins

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96ca.125 amino acids from the C-terminal region of human cleaved-LC3 (APG8b).

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Applications:
Suitable for use in Immunofluorescence, ICC, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Immunofluorescence 1:10-50
Immunocytochemistry 1:50-100

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.