MAPK14,ID (T180/Y182) (MAPK14,CSBP,CSBP1,CSBP2,CSPB1,MXI2,SAPK2A,Mitogen-activated protein kinase 14,Cytokine suppressive anti-inflammatory drug-binding protein,MAP kinase MXI2,MAX-interacting protein 2,Mitogen-activated

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-192 amino acids from the Central region of human MAPK14.

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.