P4HA3, ID (P4HA3, Prolyl 4-hydroxylase subunit alpha-3, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3)

Antibodies / Antibodies-Enzymes, Oxidase

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P4HA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-303 amino acids from the Central region of human P4HA3.

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.