CD40 (Tumor Necrosis Factor Receptor Superfamily Member 5, CD40L Receptor)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, 0.02% sodium azide.

Recombinant mouse CD40 fusion protein.

CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.

Applications:
Suitable for use in Flow Cytometry and Functional Applications. Other applications not tested.

Recommended Dilution:
Functional Application: Activates B and NK cells in vivo and in vitro. For in vivo studies use 044655 (preservative free version)
Optimal dilutions to be determined by researcher.

Endotoxin:
<0.01EU/ug (LAL test; Lonza).

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.