RANKL (TRANCE, ODF, OPGL, TNFSF11, CD254)

Antibodies / Antibodies-RANK

-20°C

Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.

Recombinant human RANKL.

RANKL is an osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. Important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Plays an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Applications:
Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (direct and indirect): 1:2000-1:10, 000
Flow Cytometry: 1:500-1:1000
Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.