SSA1 (Tripartite Motif-containing 21,52kD Ribonucleoprotein Autoantigen Ro/SS-A,52kD Ro Protein,RING Finger Protein 81,RNF81,Ro(SS-A),RO52,Sjoegren Syndrome Type A Antigen,SSA,SS-A,TRIM21,Tripartite Motif-containing Prot

Antibodies / Antibodies-Transcription Factors

-20°C

Supplied as a liquid in PBS, pH 7.2.

Full length human TRIM21, aa1-475 (NP_003132).

TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52kD Ro Protein/Ro52, and RING finger protein 81) is a 52-56kD member of the RING finger-B-box-coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475aa in length and contains one E3 ligase RING finger domain (aa16-55), a B-Box type zinc-finger region (aa92-123), a coiled-coil region (aa128-238) and a C-terminal PRY/B30.2 Ig binding domain (aa268-465). TRIM21 is reported to form trimers. Over aa195-293, human TRIM21 exhibits 72aa identity with mouse TRIM21.

Applications:
Suitable for use in Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 10ug/ml
Optimal dilutions to be determined by the researcher.

AA Sequence:
MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.