Calbindin (Calbindin D28, D-28K, Spot 35 Protein, Vitamin D-dependent Calcium-binding Protein, Avian-type, Calb1)

Antibodies / Antibodies-Calretinin

-20°C

Supplied as a liquid, 15mM sodium azide.

Prokaryotic recombinant protein corresponding to the majority of the calbindin molecule.

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It functions as a buffer of cytosolic calcium and is found in the brain, kidney, gut and pancreatic islets. In normal brain, calbindin (28kD) has been identified in medium sized neurons of the neuropil of the matrix compartment of the striatum, the woolly fiber arrangements of the globus pallidus and the fiber structures of the pars reticula of the substantia nigra. The normal expression of calbindin is modified in patients with progressive supranuclear palsy, striatal degeneration and Huntington's disease (HD). In HD, alterations to the dendritic arbors and spiny striatal neurons may be visualized by immunohistochemistry for calbindin. In moderate grades of HD, proliferative changes have been found in these areas and in severe grades, degenerative changes have been noted. A proportion of dendritic cells within the light zone of germinal centers are also noted to be positive for calbindin.

Calbindin, via the ventrolateral periaqueductal gray (vlPAG), is thought to play a role in the reactions characteristic of deep pain.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin-embeddded/frozen): 1:25-1:50 Trypsin digestion of paraffin sections may enhance staining in some cases. Incubate 60 min at 25ºC. Use standard ABC technique.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.