Ki67 (Antigen KI-67, MKI67)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid in PBS, 0.05% sodium azide.

Synthetic peptide corresponding to 62 base pair region of the human Ki-67 antigen.

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumors is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Applications:
Suitable for use in Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:20
Immunohistochemistry (Frozen): 1:20
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.