MAP1LC3A (Microtubule-associated Proteins 1A/1B Light Chain 3A,Autophagy-related Protein LC3 A,Autophagy-related Ubiquitin-like Modifier LC3 A,MAP1 Light Chain 3-like Protein 1,MAP1A/MAP1B Light Chain 3 A,MAP1A/MAP1B LC3

Pab

Bovine, Human, Mouse, Rat, Xenopus

-20°C

Supplied as a liquid in Triscitrate/ phosphate, pH 7-8, 0.09% sodium azide.

Synthetic peptide corresponding to an internal portion, aa50-125, from human LC3.

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

Applications:
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:200-1:400
Western Blot: 2ug/ml
Immunoprecipitation: 20ug/500ug of protein
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.