BCL10 (MALT-Lymphoma Marker)(B Cell CLL/lymphoma 10,B Cell leukemia/lymphoma 10,CARD-containing molecule enhancing NF-kappa-B,CARD-like apoptotic protein,caspase-recruiting domain-containing protein,CED-3/ICH-1 prodomain

Antibodies / Antibodies-Apoptosis

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Recombinant protein corresponding to aa122-168 from human BCL10.

BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1; 14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NFkappaB activation in a NIK-dependent pathway. This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1; 14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.

Applications:
Suitable for use in FLISA, Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry (Not Tested) and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Flow Cytometry (Not Tested): 0.5-1ug/10e6 cells
Immunofluorescence: 1-2ug/ml
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 1-2g/500ug protein lysate
Optimal dilutions to be determined by the researcher.

Positive Control:
WEHI-231 or Ramos cells or lymphoma

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Note: Applications are based on unconjugated antibody.