Bmi1 (B lymphoma Mo MLV insertion region (mouse),B lymphoma Mo MLV insertion region 1 homolog,Bmi 1,BMI1,BMI1 polycomb ring finger oncogene,BMI1_HUMAN,Flvi 2/bmi 1,FLVI2/BMI1,MGC12685,Murine leukemia viral (bmi 1) oncoge

Antibodies / Antibodies-Transcription Factors, Zinc (Ring) Finger

-20°C

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.2% sodium azide, 0.1mg/ml BSA, 50% glycerol.

Purified recombinant human Bmi1 protein fragments expressed in E. coli.

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A ´Lys-119´, rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.