Sox2 (ANOP3, cb236, Delta EF2a, lcc, MCOPS3, MGC148683, MGC2413, RGD1565646, Sex determining region Y box 2, SOX 2, Sox2, SOX2_HUMAN, SRY (sex determining region Y) box 2, SRY box containing gene 2, SRY related HMG box 2, SRY related HMG box gen

Antibodies / Antibodies-Transcription Factors

-20°C

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.2% sodium azide, 50% glycerol.

Purified recombinant mouse Sox2 protein fragments expressed in E. coli

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 by similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal by similarity. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation by similarity.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.