RANK (Osteoclast differentiation factor receptor)

Antibodies / Antibodies-RANK

-20°C

Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ull sterile ddH2O.

A synthetic peptide corresponding to aa29-44, QIAPPCTSEKHYEHLG at the N-terminal of human RANK.

Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is also expressed on dendritic cells and facilitates immune signaling.RANKL (Receptor Activator for Nuclear Factor κ B Ligand) is found on the surface of stromal cells, osteoblasts, and T cells. By analysis of somatic cell and radiation hybrid panels, Anderson et al. (1997) mapped the RANK gene to 18q22.1.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry (Paraffin): 0.5-1.0ug/ml. Heat induced antigen retrieval with 10mM sodium citrate buffer, pH 6.0 for 20 minutes is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.