MCAM (Melanoma Cell Adhesion Molecule, CD146, L-Gicerin, MUC18)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.

Recombinant protein corresponding to aa1-563 from mouse MCAM, NS0-derived (UniProt # Q8R2Y2).

MCAM (Melanoma cell adhesion molecule; also CD146 and MUC18) is a 110-120kD member of a small group of Ig-superfamily molecules that includes CD239 and CD166. MCAM has also been reported at a molecular weight of ~150kD. In rodent, MCAM is reportedly expressed on neurons, endothelial cells, NK cells, neutrophils, mesenchymal stem cells and melanoma cells. MCAM appears to contribute to intercellular endothelial cell junctions, and possibly contributes to the migration of select cell types. Mature mouse MCAM is a 625 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region is 540aa in length (aa24-563). It contains two V­type Ig­like domains (aa24-244) followed by three C2-type Ig­like domains (aa246-512). One cytoplasmic region splice form shows a seven aa substitution for aa600-648. Unlike human, rodent MCAM does not undergo a splicing event that will generate a soluble isoform. Over aa24-563, mouse MCAM shares 90% and 74% aa identity with rat and human MCAM, respectively.

Applications:
Suitable for use in Flow Cytometry, ELISA, and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2.5ug/10e6 cells
Immunocytochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.