TNFSF11 (Tumor Necrosis Factor Ligand Superfamily Member 11,CD254,Osteoclast Differentiation Factor,ODF,Osteoprotegerin Ligand,OPGL,Receptor Activator of Nuclear Factor kappa-B Ligand,RANKL,TNF-related Activation-induced

Antibodies / Antibodies-RANK

-20°C

Supplied as a liquid in PBS, 0.02% sodium azide.

Synthetic peptide corresponding to 14aa from human sRANK-L.

The receptor activator of NF-kB ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis. This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.5ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat Liver Tissue Lysate or Human Liver Tissue Lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.