STAT3, phosphorylated (Y705) (Signal Transducer and Activator of Transcription 3)

Antibodies / Antibodies-Transcription Factors, STAT

-20°C

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Phosphopeptide containing human STAT3 Y705.

Human STAT­3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90­-95kD member of the STAT family of transcription factors. It is 770aa in length, contains one SH2 domain (aa580­-670), and is found in almost all cell types. STAT3 mediates gp130, LIF­R, OB­R, IL10­R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo­ and hetero­dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa716­770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.