RANK-L (Receptor activator of nuclear factor kappa B,TNF-related activation-induced cytokine,TRANCE,Tumor necrosis factor ligand superfamily member 11,TNF11,TNFRSF11,Osteoprotegerin ligand,OPG,Osteoclast differentiation

Antibodies / Antibodies-RANK

-20°C

Supplied as a lyophilized powder in 0.02M PBS, pH 7.2. Reconstitute with sterile dH2O or PBS.

Recombinant protein corresponding to aa143-316 from mouse RANK-L expressed in E.coli.

RANK L is useful in studying the regulating NF-?B activation. Secreted cytokine RANKL (receptor activator of nuclear factor k-B Ligand) is critically involved in osteoclastic differentiation and activation and in the regulation of specific immunity. RANKL exists as a homotrimer, is glycosylated, and occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL (sRANKL), which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANK ligand, which is enzymatically processed by TACE (TNF-a converting enzyme (TACE; ADAM-17)). All three forms stimulate their specific receptor, RANK, which is located on osteoclastic and dendritic cells. RANKL binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Expression of RANKL is highest in the peripheral lymph nodes, weak in spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid and is up-regulated by T-cell receptor stimulation. RANKL is secreted in the soluble form.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10, 000
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.