CD147 (EMMPRIN,Neurothelin,5A11 Antigen,5F7,Basigin,Blood brain barrier HT7 antigen BSG,Extracellular Matrix Metalloproteinase Inducer (EMMPRIN),Leukocyte Activation Antigen M6,Neurothelin,OK Blood Group Antigen,TCSF,Tum

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).

Human T cell line Molt 13. Cellular Localization: Cell surface

This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Applications: 
Suitable for use in Immunofluorescence, Flow Cytometry (Not Tested) and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Not Tested): 0.5-1ug/million cells
Immunofluorescence: 1:100-1:200
Immunohistochemistry: Frozen & Formalin-fixed: 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.

Positive Control:
HSB2 cells. Colon or breast carcinoma

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.