gp100 (Melanosome,95kD melanocyte-specific secreted glycoprotein,M-beta,Melanocyte lineage specific antigen GP100,Melanocyte protein Pmel 17,Melanoma associated ME20 antigen,Melanosomal matrix protein17,p100,p26,PMEL17,P

Antibodies / Antibodies-Cancer Markers

-20°C

Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.

Extract of pigmented melanoma metastases from lymph nodes. Cellular Localization: Cytoplasmic

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Anti-HMB-45 stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

Applications: 
Suitable for use in Immunofluorescence, ELISA, Flow Cytometry (Not Tested), Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Not Tested): 0.5-1ug/million cells 
Immunofluorescence: 0.5-1ug/ml 
Western Blot: 0.5-1.0ug/ml 
Immunoprecipitation: 0.5-1ug/500ug protein lysate
Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1.0ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.

Positive Control:
SK-MEL-28 cells or Melanoma

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.