MUC18 (CD146,Cell Surface Glycoprotein MUC18,Cell Surface Glycoprotein P1H12,Gicerin,MCAM,Melanoma Adhesion Molecule,Melanoma Associated Glycoprotein MUC18,Melanoma Cell Adhesion Molecule,Melanoma-associated Antigen A32,

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).

Recombinant human MUC18 protein. Cellular Localization: Cell surface

The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Applications: 
Suitable for use in Immunofluorescence, Flow Cytometry (Not Tested), FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
FLISA: For coating, order antibody without BSA  
Flow Cytometry (Not Tested): 0.5-1ug/million cells 
Immunofluorescence: 0.5-1.0ug/ml 
Immunoprecipitation: 1-2ug/500ug protein
Western Blot: 0.5-1.0ug/ml
Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 min at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.