BCL10 (B Cell CLL/lymphoma 10,B Cell Leukemia/lymphoma 10,CARD-containing Molecule enhancing NF-kappa-B,CARD-like apoptotic Protein,caspase-recruiting domain-containing Protein,CED-3/ICH-1 Prodomain Homologous E10-like R

Antibodies / Antibodies-Apoptosis

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Human BCL10 recombinant protein (epitope aa122-168). Cellular Localization: Nuclear and cytoplasmic

BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1; 14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NFκB activation in a NIK-dependent pathway. This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1; 14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry (Not Tested), FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Not Tested): 0.5-1ug/million cells
Immunofluorescence: 1-2ug/ml
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 1-2g/500ug protein lysate
Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control:
WEHI-231 or Ramos cells or lymphoma.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Note: Applications are based on unconjugated antibody.