GFAP (Glial Fibrillary Acidic Protein, Astrocyte)

Antibodies / Antibodies-Growth Factors, Cytokines

-20°C

Supplied as a lyophilized powder in 1.2% sodium acetate, 2mg BSA, 0.2mg sodium azide. Reconstitute with 1ml PBS.

Synthetic peptide corresponding to aa341-356, EMARHLQEYQDLLNVK, from human Glial Fibrillary Acidic Protein, coupled to carrier protein BSA. Species Sequence Homology: Mouse

Glial Fibrillary Acidic Protein (GFAP) is a 50kD intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. 3 isoforms are produced from alternate splicing. These isoforms differ in the C-terminal region which is encoded by alternative exons.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry (formalin fixed/paraffin embedded tissues): 2ug/ml. Heat mediated antigen retrieval required.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.