Neurofilament Light (NF-L, NF68, NEFL, Neurofilament Light Polypeptide, NFL)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.

A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H.

Neurofilaments are composed of three intermediate filament proteins: light (~68kD), medium (~160kD) and heavy (~200kD), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10, 000-1:20, 000
Immunohistochemistry: 1:100-1:500
Immunocytochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.