Smad 2/3 (Mad3, SMAD 3, Mothers Against DPP Homolog 3, Mothers Against DPP Homolog 2, MAD-2, SMAD 2, JV18-1, MADH2, MADH3)

Antibodies / Antibodies-Smad Proteins

-20°C

Supplied as a liquid in 0.9mg sodium chloride, 0.2mg Na2HPO4, 50% glycerol.

Synthetic peptide corresponding to EQNGQEEKWCEKAVKS, from the N-terminal of human Smad2 and Smad3. Species Sequence Homology: Rat, mouse

Smad proteins are essential mediators of signal transduction by the TGF-beta superfamily from the cell surface into the nucleus. Once in the nucleus, Smad proteins target a number of DNA binding proteins to stimulate transcription. Smad4, also known as the common-mediator Smad (co-Smad), interacts with the receptor-regulated Smad proteins (R-SMAD). The R-SMAD class includes Smad2 and Smad3.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-.05ug/ml
Immunohistochemistry: 0.5-1ug/ml (formalin fixed/paraffin embedded tissues, formalin/acetone fixed tissues)
Immunohistochemistry (Frozen): 0.4-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.