STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)

Antibodies / Antibodies-Transcription Factors, STAT

-20°C

Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.

Synthetic peptide corresponding to aa686-698, YCRPESQEHPEAD, from human STAT3, coupled to carrier protein BSA.

STAT3 belongs to the STAT (signal transducers and activators of transcription) family of proteins. STAT proteins undergo tyrosine/serine phosphorylation in response to cytokines and growth factors. Phosphorylated STAT3 forms a homodimer or heterodimer with related family members such as STAT1. Phosphorylated STAT proteins are translocated to the nucleus where they act as transcription factors. STAT3 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas tissues.  Alternative splicing of the STAT3 gene gives rise to 2 isoforms.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry (formalin fixed/paraffin embedded tissues): 1-2ug/ml. Antigen retrieval required.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.