Drosophila FMR1 (Drosophila FMR1, dFMR, dFMR1, dFXR, Drosophila fragile X MentalRetardation Protein, FMRP, Fragile X Mental Retardation 1, Fragile X Mental Retardation1 Protein, fragile X Mental Retardation Protein, FRAX

Antibodies / Antibodies-Disease Markers

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

His-dFMR1 fusion protein (Drosophilia melanogaster) (C-terminal 580aa).

Fragile X syndrome is the most common inherited form of mental retardation It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein In mammals FMR1 is a member of a small protein family that consists of FMR1 FXR1 and FXR2 All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins including two KH domains and an RGG boxThe Drosophila genome contains a single gene homologous to the FXR family dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes like its human counterpart dFMR1 profile of expression recapitulates that of the human FXR protein family it is highly enriched in muscles in central nervous system and in gonads In the larval brain anti-dFMR1 also recognizes mushroom bodies a centre that mediates learning and memory These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 25ug/mg of lysate
Western Blot: Use at an assay dependent dilution. Detects a band of approximately 85kD (predicted molecular weight: 82kD)
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.