hnRNP A1 (HNRNPA 1,Helix destabilizing Protein,Helix-destabilizing Protein,Heterogeneous Nuclear Ribonucleoprotein A1,Heterogeneous Nuclear Ribonucleoprotein A1B Protein,Heterogeneous Nuclear Ribonucleoprotein B2 Protein

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Full length hnRNP-A1 native protein (partially purified) from HeLa cells (human)

This protein belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties hnRNP A1 has two repeats of quasi RRM domains that bind to RNAs It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein along with other hnRNP proteins is exported from the nucleus probably bound to mRNA and is immediately re imported Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre mRNA into hnRNP particles transport of poly A mRNA from the nucleus to the cytoplasm and may modulate splice site selection. It is also thought to have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for the hnRNP A1 gene but only two transcripts are fully described. These variant have multiple alternative transcription initiation sites and multiple polyA sites.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Use 1ug for 106 cells.
Immunohistochemistry(Paraffin): 1ug/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol
Western Blot: 1:2000-1:4000. Detects a band of ~34kD (predicted molecular weight: 38kD)
Immunoprecipitation: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.