hnRNP C1+C2 (C1,C2,Heterogeneous Nuclear Ribonucleoprotein C,Heterogeneous NuclearRibonucleoproteins C1/C2,HNRNP,hnRNP C1 / hnRNP C2,hnRNP C1/C2,hnRNPC,HNRPC,HNRPC_HUMAN,MGC104306,MGC105117,MGC117353,MGC131677,Nuclear Ri

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

RNPs eluted from oligo (dT) cellulose column

The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties hnRNP C1 and C2 are encoded by one gene the two alternatively spliced transcript variants have been described.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry(Paraffin): 1ug/ml. Perform heat mediated antigen retrieval
Western Blot: 1:1000. Detects a band of ~36kD (predicted molecular weight: 41, 43kD). Human nuclear HEK 293A lysate. Detects isoforms at molecular weight ~41, 43kD
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.