hnRNP K (CSBP,dC stretch Binding Protein,FLJ41122,Heterogeneous NuclearribonucleoProtein K,hnRNP K,HNRNPK,HNRPK,HNRPK_HUMAN,Transformation up regulated Nuclear,Transformation up regulated Nuclear Protein,Transformation u

Mab

Bovine, Human, Mouse, Rat, Xenopus

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Full length native protein (purified) (Human)

The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65kD protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, ChIP. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Detects a band of ~65kD (predicted molecular weight: 51kD)
Flow Cyt: Use 1ug for 106 cells
ChIP/Chip: Use at an assay dependent dilution. PubMed: 20673990
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.