hnRNP U (Heterogeneous Nuclear Ribonucleoprotein U, hnRNP U Protein, HNRNPU, hnRNPUProtein, HNRPU, p120, p120 Nuclear Protein, pp120, SAF A, SAF-A, SAFA, Scaffold attachment Factor A, U21.1)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

RNPs eluted from an oligo (dt) cellulose column

The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties hnRNP U is thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis this protein is cleaved in a caspase-dependent way.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, ChIP. Other applications not tested.

Recommended Dilution:
Western Blot: Use at an assay dependent dilution. Detects a band of ~97kD (predicted molecular weight: 120kD).
Immunocytochemistry/Immunofluorescence: 1:100
Flow Cytometry: 1ug for 10e6 cells.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.