Mad2L1 (Homolog of MAD2 S cerevisiae,HsMAD 2,HsMAD2,Human Mitotic Feedback Controlprotein Madp2,MAD 2,MAD 2A,MAD2,MAD2 like 1,MAD2 like Protein1,MAD2 Mitotic Arrest Deficient like 1,MAD2A,Mitotic Arrest Deficient like 1,

Antibodies / Antibodies-Disease Markers

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Recombinant protein human Mad2L1

Mad2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml. Detects a band of ~25kD (predicted molecular weight: 24.6kD).
Immunoprecipitation: 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.