MAP2 (Microtubule-Associated Protein 2, MAP 2, MAP-2) (MaxLight 550)

Pab

MaxLight 550

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Residues aa2-15 [ADERKDEGKAPHWT] of the 72kD and 280kD forms of human MAP2.

MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150, 000.

Microtubule-associated protein 2 (MAP2) is a protein that belongs to the microtubule-associated protein family. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic morphology during neural development.

Applications:
Suitable for use in Flow Cytometry (Not Tested), Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Not Tested): 1:200-1:500
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.