Neprilysin (NEP, Neutral Endopeptidase, CD10) (MaxLight 650)

Pab

MaxLight 650

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Synthetic peptide corresponding to the internal sequence of human Neprilysin.

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250, 000.

Neprilysin (NEP) is a functional neutral endopeptidase of the type that has previously been called enkephalinase or atriopeptidase. NEP mRNA levels are significantly decreased in both Alzheimer’s disease and amyloid plaques individuals. This decreased MME expression correlates with amyloid-beta deposition but not with degeneration and dementia.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.