Nestin (NES) (FITC)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

Residues aa254-270 [RATEKFQLAVEALEQEK] of 177kD human Nestin protein.

Nestin is an intermediate filament protein that is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon differentiation, Nestin is replaced by cell type-specific intermediate filaments, (e.g., neurofilaments and glial fibrillary acidic protein (GFAP)). Nestin expression is re-induced after CNS injury and during regeneration of injured muscle tissue.

Applications:
Suitable for use in Flow Cytometry (Not Tested), Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Not Tested): 1:200-1:500
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.