Mitofusin-1,NT (Fzo Homolog,Hfzo1,Hfzo2,MFN 1,Mfn1,MFN1_HUMAN,Mitochondrial Transmembrane GTPase Fzo 1,Mitochondrial Transmembrane GTPase FZO 2,Mitochondrial Transmembrane GTPase FZO1B,Mitofusin 1,Mitofusin1,Putative Tra

Antibodies / Antibodies-GTPase, Rab, Ras, Rho Proteins

-20°C

Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.

Synthetic peptide corresponding to aa23-49, DQLLEFVTEGSHFVEATYKNPELDRIA, from human Mitofusin 1 at N-terminal, different from the related mouse and rat sequences by 1aa.

Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80­90kD mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN­2, and to undergo ubiquitination by MARCH5. MFN­1 has two key domains. One is a coiled­coil region that mediates MFN­1: MFN­1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.