MAP1LC3B, NT (LC3B, Microtubule-associated protein 1 light chain 3 beta, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1ALC3, Autophagy-related protein LC3 B)

Antibodies / Antibodies-Microtubule-associated Proteins

-20°C

Supplied as a liquid in PBS, 0.02% sodium azide.

Peptide corresponding to 18aa near the N-terminal of human MAP1LC3B.

Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 20ug/mL.
Western Blot: 1-2ug/ml.
Immunohistochemistry (paraffin): 5ug/mL
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Brain Tissue Lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.