ACE2 (Angiotensin I Converting Enzyme 2, ACEH, ACEII, ACE-II, Peptidyl-Dipeptidase A, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15)

Antibodies / Antibodies-ACE

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Leu392-Val739 of rat ACE2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin. ACE 2 has direct effects on cardiac function, and is expressed predominantly in vascular endothelial cells of the heart and the kidneys. By EST database searching for sequences showing homology to the zinc metalloprotease angiotensin-I converting enzyme (ACE) and by screening a human lymphoma cDNA library, Tipnis et al. (2000) cloned a full-length ACE2 cDNA, which they called ACEH, encoding a deduced 805aa protein that shares approximately 40% identity with the N- and C-terminal domains of ACE. ACE2 contains a potential 17aa N-terminal signal peptide and a putative 22aa C-terminal membrane anchor.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.