Bcl10 (B-Cell CLL/Lymphoma 10, CARMEN, CIPER, CLAP, c-E10, mE10, CARD-containing molecule enhancing NF-kappa-B, CED-3/ICH-1 prodomain homologous E10-like regulator, Cellular homolog of vCARMEN)

Antibodies / Antibodies-Apoptosis

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Met1-Gln233 of human Bcl10, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. B-cell lymphoma/leukemia 10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.