CRN (Corin, ATC2, CRN, Lrp4, TMPRSS10, Atrial Natriuretic Peptide-Converting Enzyme, Serine Protease, Heart-specific serine proteinase ATC2, Pro-ANP-converting enzyme)

Antibodies / Antibodies-Enzymes

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Gln334-Ser477 of mouse CRN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

Corin encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase.
The corin cDNA encodes a deduced 1042aa, type II transmembrane protein. At its N terminus, corin contains a short cytoplasmic domain and a transmembrane sequence. In the extracellular region of corin, there are 2 frizzled-like cysteine-rich motifs, 7 low density lipoprotein receptor repeats, a macrophage scavenger receptor-like domain, and a trypsin-like protease domain at the C terminus.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QSLCGGG ESFLCTSGLC VPKKLQCNGY NDCDDWSDEA HCNCSKDLFH CGTGKCLHYS LLCDGYDDCG DLSDEQNCDC NLTKEHRCGD GRCIAAEWVC DGDHDCVDKS DEVNCSCHSQ GLVECRSGQC IPSTFQCDGD EDCKDGS

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.