ERAP2 (Endoplasmic Reticulum Aminopeptidase 2, L-RAP, LRAP, Leukocyte-Derived Arginine Aminopeptidase)

Antibodies / Antibodies-Amyloid

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Pro41-Phe271 of human ERAP2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.

Aminopeptidases hydrolyze N-terminal aa of proteins or peptide substrates. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP1 and LRAP to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II (TPP2) The deduced full-length protein contains 960aa and has a calculated molecular mass of 123kD. It shares 43% and 49% identity with PLAP and ERAP1, respectively, and contains a hydrophobic N-terminal region, zinc metallopeptidase (M1) motifs, and 9 potential N-glycosylation sites. The truncated form, LRAP(s), contains 532aa. Northern blot analysis using the full-length LRAP cDNA as probe detected ubiquitous expression of a 6.7-kb transcript, with relatively high expression in spleen and leukocytes.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
ELISA: 1:100-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.