GCNF (Germ Cell Nuclear Factor, NR6A1, NR6-A1, 6,Group A,Member 1, Retinoid receptor-related testis-specific receptor)

Antibodies / Antibodies-Neuroscience

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Glu282-Glu480 of human GCNF, two N-terminal Tags, His-Tag and T7-tag expressed in E. coli.

The germ cell nuclear factor (GCNF) protein, also known as RTR (retinoid receptor-related testis-associated receptor), is a member of the nuclear receptor family of intracellular transcription factors and is encoded by the NR6A1 gene (nuclear receptor subfamily 6, group A, member 1).In adults, GCNH is expressed mainly in the germ cells of gonads and is involved in the regulation of embryogenesis and germ cell differentiation.
Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. The gene expresses three alternatively spliced transcript variants.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.