GFAP (Glial Fibrillary Acidic Protein, Intermediate Filament Protein)

Antibodies / Antibodies-Neuroscience

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Trp253-Leu354 of mouse GFAP, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.

GFAP is an intermediate filament (IF) protein that is found in glial cells such as astrocytes. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP helps to maintain astrocyte mechanical strength, as well as the shape of cells. GFAP is expressed in the central nervous system in astrocyte cells. It is involved in many cellular functioning processes, such as cell structure and movement, cell communication, and the functioning of the blood brain barrier.
The predicted amino acid sequence indicated that GFAP shares structural similarities--particularly in the central rod domain and to a lesser degree in the carboxyl-terminal domain--with other IF proteins found in nonepithelial cell types.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.