MAP1A (Microtubule Associated Protein 1A, MAP-1A, MAP1-A, MAP1L, MTAP1A, Proliferation-related protein p80)

Antibodies / Antibodies-Microtubule-associated Proteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Glu2110-Ser2367 of rat MAP1A fused to His-Tag and T7-tag at N-terminal expressed in E. coli.

These proteins have been divided into 2 main groups by molecular mass, high molecular weight MAPs, which include MAP1A, MAP1B, and MAP2, and another group of intermediate-sized proteins, which include the abundant tau MAPs. MAP1B, also named MAP5, is a component of long cross-bridges between microtubules and is a filamentous molecule with a small spherical segment at one end.
Lien et al. (1994) completely cloned and sequenced the human MAP1B gene. By comparison of human MAP1B with sequence databases, they identified a MAP1B-related gene that is probably the human homolog of rat MAP1A. The human MAP1A gene is expressed at high levels in brain and spinal cord and at much lower levels in muscle.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.