NAT1 (N-acetyltransferase 1, AAC1, Arylamine N-Acetyltransferase, Arylamide acetylase 1, Monomorphic arylamine N-acetyltransferase)

Antibodies / Antibodies-Enzymes, Acetyltransferase

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Arg9-Glu254 of mouse NAT1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

NAT1 is one of two arylamine N-acetyltransferase (NAT) genes in the human geneome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. NAT2 gene is the site of the polymorphism that was first identified through 'isoniazid inactivation' and is also known as 'acetylator phenotype.' The NAT1 gene is responsible for the N-acetylation of certain arylamine drugs such as p-aminosalicylic acid and shows no variability, i.e., is monomorphic.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.