PBRM1 (Polybromo 1, PB1, BAF180, BRG1-associated factor 180)

Antibodies / Antibodies-Proteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Gly1152-Gln1273 of human PBRM1, fused to N-terminal His-Tag expressed in E. coli.

The deduced 1, 582aa BAF180 protein, which is 90% identical to the chicken polybromo-1 protein, possesses 5 bromodomains, 2 bromo-adjacent homology (BAH) regions, and 1 high-mobility group (HMG) motif. BAF180 is also highly homologous to yeast Rsc1, Rsc2, and Rsc3, essential proteins that are required for cell cycle progression through mitosis.
Northern blot analysis revealed wide expression of 9.5- and 7.5-kb BAF180 transcripts. Immunofluorescence microscopy demonstrated that BAF180 was expressed in the nucleus during interphase but colocalized with cytoplasmic dynein at some kinetochores during prometaphase. The localization of BAF180 at kinetochores was not observed in metaphase, anaphase, or telophase.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.