SCARD1 (Scavenger Receptor Class D Member 1, CD68, GP110, Macrosialin, Macrophage Antigen CD68)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Ala27-Leu303 of mouse SCARD1, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.

CD68 is a 110kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. Holness and Simmons (1993) isolated cDNA clones encoding CD68 by transient expression in COS cells and immunoselection using anti-CD68 monoclonal antibodies. Two types of cDNA clones were isolated, differing by the insertion of 2 short segments in the N-terminal portion of the extracellular domain. The cDNA sequence predicted a type I integral membrane protein of 354 residues with a heavily glycosylated extracellular domain of 298 residues containing 9 potential N-linked glycosylation sites and numerous potential O-linked glycosylation sites.CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
ELISA: 1:100-1:5000
Optimal dilutions to be determined by the researcher.

AA Sequence:
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-AVTL LPSFTMTPTA TESTASPTTS HRPTTTSHGN VTVHTSSGPT TVTHNPATTT SHGNATISHA TVSPTTNGTA TSPRSSTVGP HPGPPPPSPS PRSKGALGNY TWANGSQPCV QLQAQIQIRI LYPIQGGRKA WGISVLNPNK TKVQGGCDGT HPHLSLSFPY GQLTFGFKQD LHQSPSTVYL DYMAVEYNVS FPQAAQWTFM AQNSSLRELQ APLGQSFCCG NASIVLSPAV HLDLLSLRLQ AAQLPDKGHF GPCFSCNRDQ SLLLPLIIGL VLL

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.