SYNC (Syncoilin, Syncoilin-1, Intermediate Filament Protein, Syncoilin intermediate filament 1)

Antibodies / Antibodies-Cardiac Markers

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Ile169-Pro458 of human SYNC, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.

Syncoilin is a muscle-specific intermediate filament, first isolated as a binding partner to alpha-dystrobrevin, as determined by a yeast two-hybrid assay.Later, a yeast two-hybrid method was used to demonstrate that syncoilin is a binding partner of desmin. These binding partners suggest that syncoilin acts as a mechanical "linker" between the sarcomere Z-disk (where desmin is localized) and the dystrophin-associated protein complex (where alpha-dystrobrevin is localized). However, the specific in vivo functions of syncoilin have not yet been determined. Abnormally high levels of syncoilin have been shown to be a characteristic of neuromuscular wasting diseases, such as desminopathy and muscular dystrophy. Therefore, syncoilin is being explored as a promising marker of neuromuscular disease.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.