TUBb (Tubulin Beta, TUB-B, TUBB5, TUBB1, M40)

Antibodies / Antibodies-Tubulin

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Val170-Val419 of human TUBb, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

To form microtubules, the dimers of alpha- and Beta-tubulin bind to GTP and assemble onto the ends of microtubules while in the GTP-bound state. After being incorporated into the microtubule, the bound molecule of GTP will hydrolyse into GDP. Although both subunits bind GTP, only the Beta-subunit has GTPase activity; that is, Beta-tubulin can hydrolyse GTP to GDP whereas alpha-tubulin cannot. Whether the Beta-tubulin member of the tubulin dimer is bound to GTP or GDP influences the stability of the dimer in the microtubule. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart; thus, this GTP cycle is essential for the dynamic instability of the microtubule. Class III Beta-tubulin‎ is a microtubule element expressed exclusively in neurons, and is a popular identifier specific for neurons in nervous tissue.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.