VTN (Vitronectin, V75, VN, VNT, Serum Spreading Factor, Somatomedin B, Complement S-Protein)

Antibodies / Antibodies-Vitronectin

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Gln21-Arg400 of rat VTN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

Vitronectin, is a 75kD glycoprotein in plasma and tissue. (S-protein is not to be confused with protein S.) A multifunctional protein, it promotes attachment and spreading of animal cells in vitro, inhibits cytolysis by the complement C5b-9 complex, and modulates antithrombin III-thrombin action in blood coagulation. The primary structure of vitronectin has been deduced from the sequence of its cloned cDNA. Polymorphism of vitronectin of plasma has been demonstrated. Sun and Mosher (1989) demonstrated that the frequencies are different in Orientals and Caucasians. By use of high resolution fluorescence in situ hybridization (FISH), Fink et al. (1992) mapped the VTN gene to 17q11. The localization was confirmed by cohybridization with a centromere-specific alphoid probe.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.