XPD (Xeroderma Pigmentosum, Complementation Group D, CXPD, BTF2 p80, EM9, TTD, ERCC2, TFIIH basal transcription factor complex 80kD, De Sanctis-Cacchione, Excision Repair Cross-Complementing Rodent Repair Deficiency 2)

Antibodies / Antibodies-Transcription Factors

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to Ala404-Leu637 of human XPD, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.

ERCC2, or XPD is a protein involved in transcription-coupled nucleotide excision repair. The XPD (ERCC2) gene encodes for a 2.3-kb mRNA containing 22 exons and 21 introns. The XPD protein is a 760aa polypeptide with a size of 87kD. Defects in this gene can result in three different disorders: the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Just like XPB, XPD is also a part of human transcriptional initiation factor TFIIH and has ATP-dependent helicase activity. It belongs to the RAD3/XPD subfamily of helicases. XPD is essential for the viability of cells. Deletion of XPD in mice is embryonic lethal.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Immunocytochemistry: 1:50-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.